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McFadeyan Reaction
The McFadyean reaction is a staining technique used to detect the capsule of Bacillus anthracis directly in blood or tissue smears.
Developed by Sir John McFadyean in 1903.
Bacillus anthracis has a polypeptide capsule made of poly-D-glutamic acid, which:
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Is not visible with standard Gram stain
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Can be selectively stained using polychrome methylene blue (PMB)
The capsule appears as a pink to purplish halo around blue-stained bacilli.
Snapshot
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