1950 – L. J. Elek introduced the in vitro precipitation test for determining toxin production by C. diphtheriae. This became known as Elek’s test.
Elek’s test is based on immunodiffusion:
Diphtheria toxin + diphtheria antitoxin → visible precipitin line
A strip of filter paper containing diphtheria antitoxin is placed on an agar plate. The suspected C. diphtheriae strains are streaked perpendicular to the strip.
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